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Equipment

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Overview equipment

A click on the device picture will lead you to the manufacturer device specification webpage.

Sequencer

MiSeq

NGS Sequencer (Illumina) for smaller sequencing projects:

  • Targeted sequencing: Gene Panels up to 10 Mb
  • miRNA and small RNA Analysis
  • 16S Metagenomic Sequencing
  • Maximum output of 15 Gb per run
  • Sequencing length up to 2 x 300bp

NextSeq500

NGS Sequencer (Illumina) for small to mid -size Projects:

  • Targeted sequencing: Gene Panels up to 10 Mb
  • RNA-seq or ChIP-seq: typically 7-8 Samples
  • Maximum output of 120 Gb per run

HiSeq 4000

NGS Sequencer (Illumina) for mid to large -size Projects:

  • Genome Sequencing of <1Gbp-large genomes
  • Targeted resequencing: Gene Panels, Exome (e.g. 110x coverage: 48 samples/run)
  • RNA-seq and ChIP-seq, Methyl-seq
  • Max of 1500 Gb per run (8 lanes)

Quality Control

Agilent 4200 TapeStation

Automated system for QC of up to 96 samples:

  • Integrity of genomic DNA or total-RNA
  • NGS-libraries: fragment size and concentration analysis
  • Sample-QC for Micro arrays (aCGH) or quantitative PCR (qPCR)

Agilent 2100 Bioanalyzer

Abbildung eines Bioanalyzers 2100

Manual system for QC of samples:

  • evaluation of fragment size distributions for several types of samples (DNA, RNA).

Thermo Fisher Qubit 3.0

Fluorometer for the quantitation of DNA, RNA, microRNA, and protein using highly sensitive and accurate fluorescence-based quantitation assay.

Thermofisher NanoDrop One

Micro-volume spectrophotometer for sample QC.

ROCHE LightCycler 480 II

High-throughput PCR platform (96- or 384-well plates) that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation.

Automatisation

Agilent NGS Workstation (Option B)

Liquid handling platform enabling automatization of NGS protocols.

Beckman Coulter - Biomek i7

pipetting robot for high throughput automation

Brand - Liquid Handling pipetting robot

Automatic pipetting system for smaller samples sizes

Thermo - KingFischer Flex

Automized purification system (DNA/RNA/protein)

Single cell sequencing

10x Genomics Chromium system

Flexible Single Cell solution

Fragment Size Selection

Sage Science - PippinHT

Fragement size selection for Next-Gen Sequencing